Umgaqo-siseko we-automatic nucleic acid extraction kunye nesixhobo sokucoca

I-automatic nucleic acid extraction kunye nezixhobo zokuhlanjululwa kukukhupha kunye nokucoca i-nucleic acid ngendlela ye-magnetic bead, ngokokukhethwa kwekiti ehambelanayo yeesampuli zemithombo emininzi (ezifana negazi, izilwanyana kunye nezityalo, iiseli, njl. njl.) kunye nokuhlanjululwa. Isixhobo sinomsebenzi ozinzile, ingxolo ephantsi, ukusebenza ngokuzenzekelayo, ngokukhawuleza kwaye kulula, kwaye inokucoca iisampuli ze-1-32 ngexesha elifanayo.
Umgaqo wokusebenza:
Emva kwesampulu ye-lysis, iimolekyuli ze-nucleic acid ezikhutshiweyo zibhengezwa ngokuthe ngqo kumphezulu wamaso kazibuthe, kwaye iimolekyuli ze-nucleic acid ziyanyibilika kwindawo edlulayo ngokufunxa kazibuthe, ukudluliselwa kunye nokuhlamba ngeentonga zemagneti ezakhelwe ngaphakathi. Ngeentlobo ezahlukeneyo zamaso kazibuthe i-nucleic acid reagents, i-nucleic acid esulungekileyo inokufumaneka.
Imowudi yokunyakaza kwebar magnetic: imotor yokunyathela esetyenziswa kumazwe ngamazwe isetyenziswa njengesixhobo sokuqhuba, kwaye umkhono wemagnethi webar unokulungelelanisa ngokuzenzekelayo i-vibration amplitude ngokomthamo wesisombululo, ukuze isampulu ixutywe ngokupheleleyo; Ukusebenzisa isixhobo sokuhambisa isikrufu sebhola, umkhono wentonga kazibuthe kunye nentonga kazibuthe isebenza ngokuzinza, ukuchaneka okuphezulu, ukwandisa ubomi benkonzo; Inxalenye nganye ehambayo ixhotyiswe ngomsebenzi wokukhusela isikhundla ukuphepha ngokufanelekileyo ukungaphumeleli.
Imowudi ye-adsorption eyomeleleyo: ngemowudi esanda kuyilwa eyomeleleyo yobhengezo, amaso kazibuthe ancanyathiselwa kwintloko yentonga kazibuthe, ukuze kuqinisekiswe ukuba umbane usenakho ukugquma onke amaso kazibuthe naxa umthamo we-elution umncinci kakhulu. Amaso kazibuthe anesiphumo esingcono sokubhengeza kunye nesivuno esiphezulu se-nucleic acid.

I-automatic nucleic acid extraction kunye nezixhobo zokucoca:
I-1, isikrini sokuchukumisa isikrini: i-intshi ye-10.1 yesikrini esikhulu esinombala we-interface yesiShayina, ukusebenza komntu ochukumisayo, uyilo olufanelekileyo lweqhosha, kulula ukuyisebenzisa;
2, uyilo lwesampulu lukhethekile: uyilo lwendawo yepleyiti eyenziwe ngabantu, ikhululekile ukuthatha iimpahla, ukunqanda ungcoliseko kunye neengozi ezibangelwa yimiqobo yokusebenza;
I-3, umsebenzi wenkqubo onamandla: unokwenza ngokwezifiso ubushushu, inkqubo ekhawulezayo, isitshixo esinye sokuqalisa, sinokuhlangabezana neemfuno zee-reagents ezahlukeneyo;
I-4, i-nucleic acid extraction fast: ixesha elifutshane lokusebenza, i-15-39min inokugqiba iisampuli ze-32 zokukhutshwa (kuxhomekeke kwi-reagent);
I-5, inkqubo yokusasazwa kwelizwi: abasebenzisi abakhawulezayo belizwi banokulawula inkqubo ngokwendlela yokulawula esemgangathweni, kwaye ikhuthaze ukugqitywa kwemisebenzi yovavanyo;
6, izinga eliphezulu recovery: nucleic acid recovery & GT; 95%, ukubuyiswa bead magnetic & GT; 98%;
I-7, ukulawulwa kokuchasana nokungcoliseka: uyilo oluvaliweyo ngokupheleleyo, ixesha elakhelwe ngaphakathi kwi-UV disinfection, i-fan exhaust, ukuphepha ngokufanelekileyo ukungcoliseka kwe-aerosol, i-alarm anti-door isebenza, qinisekisa ukhuseleko lokusebenza;
8, inkqubo yokusebenza ekrelekrele: usebenzisa inkqubo yokusebenza ye-Android, unokukhangela ngokukhawuleza Izicwangciso zenkqubo, isilayidi ukukhetha iinkqubo, utshintshe iinkqubo ezilula ngakumbi.
Ukusetyenziswa kunye nokugcinwa kwe-oven yokomisa
Ukomisa i-oveni ilungele ubuthathaka bobushushu, kulula ukubola kunye nezinto zokomisa i-oxidative, zisetyenziswa ngokubanzi kwi-electronics, iibhetri, isinyithi, iplastiki, unxibelelwano, iikhemikhali zokugquma, izixhobo zemoto kunye nezithuthuthu, i-epoxy resin, izinto zokuthambisa zokuthambisa, izixhobo zemagneti kumashishini oshishino. , iiyunivesithi kunye neekholeji, uphando lwezenzululwazi kunye nelabhoratri enxulumene nezinto, ukomisa, ukubhaka, ukunyibilika kwewax kunye nokuvala inzala.
Ukusetyenziswa kwe-oven yokomisa:
Umsebenzisi kufuneka afunde imiyalelo ngononophelo kwaye aqhelane nemisebenzi ye-incubator phambi kokuba avule umbane. Tshintshela unikezelo lwamandla, cinezela iswitshi yamandla, isalathisi samandla sivuliwe. Lungisa isilawuli seqondo lokushisa kwiqondo lokushisa elifunekayo ngumsebenzisi. Xa iqondo lokushisa elibonisa i-incubator lifikelela kwiqondo lokushisa elimiselweyo, ukufudumeza kuphazamisekile kwaye isalathisi sokufudumala sivaliwe. Emva kwemizuzu engama-90 yamandla kwiqondo lobushushu eliqhelekileyo le-ambient, ubushushu bunokuhlala buzinzile. Ukuba iqondo lokushisa elikhawulezayo kwi-incubator lidlula i-alarm ephezulu esetyenzisiweyo, isalathisi sokulandelela iqondo lokushisa lomlawuli weqondo lokushisa livuliwe, kwaye ukunikezelwa kwamandla okufudumeza kunqunyulwa ngokuzenzekelayo. Ukuba umnyango weglasi uvulwe ukuthatha iisampulu, isifudumezi kunye nomatshini womoya ojikelezayo uya kuyeka ukusebenza. Xa ucango lweglasi luvaliwe, isifudumezi kunye nefeni zinokusebenza ngokuqhelekileyo, ukuze kuthintelwe ukungcoliseka kwenkcubeko kunye nokugcwala kwamaqondo obushushu.
Ukugcinwa kunye nokugcinwa kwe-oven yokomisa:
Gcina ubuso be-incubator bucocekile kwaye buhle. I-incubator kufuneka ifakwe kwigumbi elinomoya, kwaye izinto ezinokutsha kunye neziqhushumbisi akufanele zibekwe ngeenxa zonke. Musa ukubeka izinto ebhokisini zixinene kakhulu, kufuneka ushiye indawo. Ingaphakathi nangaphandle kwebhokisi kufuneka igcinwe icocekile. Emva kokusetyenziswa ngalunye, kufuneka icocwe. Ukuba ingasetyenziswanga ixesha elide, kufuneka igutyungelwe ngesigqubuthelo sothuli kwaye ifakwe kwigumbi elomileyo. Abasebenzi bolawulo lwezixhobo baya kuqhuba ungqinisiso lwemitha ngokwesicwangciso sokuqinisekisa kwaye bajonge ulawulo lobushushu ngamaxesha athile. Xa amaqondo obushushu akwindawo esezantsi engaphantsi kwama-40 ° C ehlotyeni, sebenzisa i-air conditioner ukuthoba iqondo lobushushu kwindawo (25-28 ° C ebusuku) ukunqanda ukwehla kobushushu. Musa ukubeka i-incubator kwindawo yokushisa ephezulu okanye kwindawo emanzi kwaye ugweme ukukhanya kwelanga. I-fan kwi-incubator ihlala igcwele igrisi yokuthambisa. Ukuba ingasetyenziswanga ixesha elide, amanzi ebhantshi lamanzi kufuneka akhululwe, kwaye igrisi engathathi hlangothi okanye i-vaseline kufuneka ifakwe kwiindawo ze-electroplated ukukhusela ukubola. Isigqubuthelo sothuli lweplastiki kufuneka sibekwe ngaphandle kwe-incubator, kwaye i-incubator kufuneka ifakwe kwigumbi elomileyo ukuze ugweme umonakalo omanzi kumlawuli wokushisa.
Iimpawu zeMveliso:
1. I-studio yeCuboid, ukwandisa umthamo wokusetyenziswa.
I-2, isixhobo esikhethekileyo sokuqinisa izixhobo, ngokusetyenziswa kwe-liner yentsimbi ejiyileyo, ukuqinisekisa ukusetyenziswa kwexesha elide kwezixhobo ze-liner deformation.
3, ucango lweglasi oluqiniweyo, ukutywinwa kakuhle, jonga izinto ezikwigumbi lokusebenza, ngokukrwaqula.
I-4, yonke i-silicone yerabha yomnyango wokutywina umsesane, ukuqinisekisa ukusebenza okugqwesileyo kokutywinwa kwezixhobo.
I-5, ukulawulwa kweqondo lokushisa usebenzisa amaqhosha okuthintela umboniso wedijithali, ukusetwa kwedijithali, umboniso wedijithali kunye ngqo, ukufudumeza ukulawula ukushisa, ukupholisa, inkqubo ezimeleyo ngokupheleleyo inokuphucula ukusebenza kakuhle, ukunciphisa iindleko zokuvavanya, ukwandisa ubomi, ukunciphisa izinga lokungaphumeleli.
6, ubushushu besixhobo ngokubanzi; Izixhobo zizonke ziphantsi kwesigaba/isigaba esiguquliweyo; Ukugcwala kwezixhobo zizonke; Ixesha lilonke izixhobo;
7, okunye ukuvuza, imiyalelo yokusebenza, ukuvala ngokuzenzekelayo emva kokungaphumeleli kokukhusela i-alarm.


Ixesha lokuposa: Mar-13-2022