CFX96TOUCH Fluorescent Quantitative PCR isixhobo

I-CFX96Touch fluorescent quantitative PCR ingasetyenziswa kwiinkalo ezahlukeneyo zophando lwe-nucleic acid quantification, uhlalutyo lwenqanaba lokuchazwa kofuzo, ukubhaqwa kokuguqulwa kofuzo, ukubhaqwa kwe-GMO, kunye nohlalutyo oluthile lwemveliso.

Indawo yokusebenza:
1.1 Ubushushu bokusebenza: 5-31 ° C
1.2 Ukusebenza kunye nokufuma: ukufuma okuhambelanayo ≤80%
1.3 Amandla okusebenza: 100-240 VAC, 50-60Hz
Ukusebenza neemfuno zobugcisa zeCFX96Touch fluorescence quantitative PCR
3.1 Umsebenzi ophambili (* sisalathi ekufuneka sihlangatyezwe)
* 3.1.1 Iziteshi zokuvavanya ezithandathu, i-5% ye-PCR inokuqondwa, kwaye i-5 i-gene ejoliswe kuyo inokubonwa ngexesha elifanayo, kwaye i-channel ekhethekileyo yokufumanisa i-FRET ifunyenwe ngaxeshanye.
* 3.1.2 Ngomsebenzi oguqukayo weqondo lobushushu lePCR, unokubaleka amaqondo obushushu ahlukeneyo asi-8 ngaxeshanye, ufukamo lobushushu ngalinye.
3.1.3 Ukuvulwa kwee-reagents ezipheleleyo, uphando olwahlukeneyo kunye neerejenti zeklinikhi ziyasebenza;
3.1.4 Ifanelekile kwiindlela ezahlukeneyo ze-fluorescence ezifana ne-Taqman, i-Molecular Beacon, i-Fret probe, i-Sybr Green i, njl.
3.1.5 Vula, 0.2ml ityhubhu eyodwa, i-octal, i-96-well plate, njl.
* 3.1.6 inokusebenza ngokuzimeleyo, ukusebenza ngaphandle kwe-intanethi ngokwenene, akukho mfuneko yokudibanisa ikhompyutha ukubeka iliso kwijika le-PCR le-fluorescent amplification ngexesha lokwenyani;
3.2 Iimfuno eziphambili zobugcisa (* sisalathi ekufuneka sifezekiswe)
* 3.2.1 Umthamo wesampuli: 96 × 0.2ml, iinkcukacha eziqhelekileyo ze-96-well plates (12 × 8) zingasetyenziswa;
3.2.2 Uhlobo lweeMbonelelo: I-0.2ml ityhubhu enye, i-eight interlocking, i-96-well plates, njl.
3.2.3 Inkqubo yokuphendula: 1-50μL (icetyiswa i-10-25 μL);
* 3.2.4 Umthombo wokukhanya: Ii-LED ezintandathu ezinezihlungi;
* 3.2.5 Umtshina: Iidiode ezintandathu ezinezihluzi;
* 3.2.6 ilitha yokupholisa isantya: 5 ° C / isekhondi;
3.2.7 Uluhlu lokulawula ubushushu: 0 -100 ° C;
3.2.8 Ukuchaneka kobushushu: ± 0.2 ° C (90 ˚C);
3.2.9 Ubushushu obufanayo: ± 0.4 ° C (90 ˚C kwimizuzwana eyi-10);
* 3.2.10 Iqondo lobushushu eliGunyazayo Umsebenzi weGradient: Baleka amaqondo obushushu ahlukeneyo ayi-8 ngexesha elinye; Uluhlu lokulawula ubushushu be-gradient: 30 -100 ° C; iqondo lobushushu umahluko uluhlu: 1 – 24 ° C; ixesha lokufukamela lobushushu begradient: kuyafana;
I-3.2.11 i-excitation / emission wavelength range: 450-730 nm;
3.2.12 Uvakalelo: Ikopi enye yemfuza kwigenome yomntu inokubonwa;
3.2.13 Uluhlu lweDynamic: 10 amanani;
3.2.14 Bonisa: 8.5 intshi umbala touch screen;
3.2.15 Imowudi yoHlalutyo lweDatha: Ubungakanani boMgangatho weCurve, i-Melting Curve, i-CT okanye i-ΔCT CT Gene Expression Analysis, i-Multiple Internal Genodes Analysis kunye ne-Amplification Efficiency Calculation, iiFayile ezininzi zeDatha yeGene Expression Analysis, i-Allelic Analysis, uHlalutyo loHlobo, i-Gene, umsebenzi wokuhlalutya i-curve. ;
3.2.16 Ukuthunyelwa ngaphandle kwedatha: Excel, Word, okanye PowerPoint. Ingxelo yomsebenzisi iqulethe useto lokuqhuba, imizobo, kunye neziphumo zedatha yetafile, ezinokuprintwa okanye zigcinwe njengePDF;
* 3.2.17 Izifundo zesakhiwo seChromosomal: Indlela yokuhlalutya ubungakanani bezakhiwo zechromatin ngendima yokuthelekisa ukuthotywa kwe-genomic DNA ngokuthelekisa indima ye-genomic DNA. Ingqina ngokwenyani ukuhambelana kobude phakathi kolwakhiwo lwechromatin kunye nokubonakaliswa kofuzo;