5425R High-speed Frozen Centrifuge

Amen's 5425R high-speed frozen centrifuge (Centrifuge5425R), uhlobo iqhosha, uhlobo umkhenkce, rotor FA-24X2, 230V / 50-60Hz (CN), ujongano lokusebenza iqhosha, ukucoca olufanelekileyo. Umkhenkce.

Amen's 5425R high-speed frozen centrifuge (Centrifuge5425R), uhlobo iqhosha, uhlobo umkhenkce, rotor FA-24X2, 230V / 50-60Hz (CN), ujongano lokusebenza iqhosha, ukucoca olufanelekileyo. Umkhenkce. Njengemveliso elandelayo ye-Centrifuge 5424 / 5424R eyaziwayo, le mizekelo mibini emitsha ibandakanya zonke iimveliso ozithandayo iimodeli zangaphambili, kunye ne-rotors entsha kunye neempawu zesofthiwe, okwenza umsebenzi wakho wemihla ngemihla usebenze ngakumbi kwaye ujabule. Uyilo olukhethekileyo lwe-Centrifuge5425 epholileyo yomoya iqinisekisa ukusebenza okuthe cwaka ukubonelela ngendawo yokusebenza ekhululekile. Ukuya kuthi ga kwimizuzu eli-12, i-centrifugation inokufezekiswa, kwaye uyilo lokuthintela isandi luyasetyenziswa, kwaye ingxolo eqhelekileyo ngama-45dB (a). Le misebenzi yexesha elifutshane yanele ukugqiba amanyathelo e-centrifugation yezona zixhobo eziqhelekileyo zokucoca i-DNA / RNA. Xa usebenza ixesha elide, i-fan yokupholisa ichithwa ngokufanelekileyo ngexesha lomsebenzi oseleyo.

Iinkqubo zokupholisa ze-Centrifuge5425R eziphuculweyo ziqinisekisa isiphumo esihle sokwahlula, nakwisantya esiphezulu se-rotor, inokuthenjwa ngokuthembekileyo kwi-4 ° C. Ixesha elifutshane nje lemizuzu eyi-8 ngaphambi kokupholisa (~ 21 ° C ukuya kwi-4 ° C), i-centrifuge inako. zisetyenzwe nangaliphi na ixesha. Iirotha ezahlukeneyo ngoku zigubungela uluhlu olubanzi lwezicelo, kubandakanya usetyenziso lwebhayoloji yemolekyuli, efana neDNA / RNA okanye ukucocwa kweprotein, imvula yeebhaktheriya, ikhuliswe ukusuka kwi-5 ml yeetyhubhu zovavanyo, kunye neeseli, kunye novavanyo lokulandela umkhondo, umzekelo, iPCR precipitation. -unyango / emva kokunyanga kunye nenkqubo ye-QPCR yakha kwi-tube ye-0.2 ml, i-plate ye-8-well plate, okanye i-segmented 96-well plate. I-Eppendorfkitrotor® (FA-18X2-Kit) isetyenziselwa i-18 x centrifugal columns kunye ne-1.5 / 2.0ml centrifuge tubes (ubuninzi be-18,565 xg). Le rotor ekhethekileyo isombulula iingxaki eziqhelekileyo ezidibana nazo xa usebenzisa ikhithi yekholamu ejikelezayo ye-Miniprep: Isigubungelo sesikhongozeli esivulekileyo sinqunyulwe kwinyathelo lokuluting. Ngokuqhelekileyo kwenzeka ntoni le ngxaki kukuba i-rotors yendabuko ayikwazi ukubonelela ngenkxaso eyaneleyo ye-centrifuge caps evuliwe ngexesha le-centrifugation. I-Eppendorfkitrotor inomda ophakanyisiweyo obekwe kuyo ngexesha le-centrifugation, ngoko abayi kunqunyulwa. Ukusukela ngoku ukuya phambili, isigubungelo sakho sesikhongozeli siya kuhlala sikwindawo yayo. I-rotor FA-10 × 5 isetyenziswe kwi-10 × 5.0mleppendorf centrifuge tubes (ukuya kwi-21,300 xg), kunye neebhaktheriya, igwele lenkcubeko okanye iiseli zivunwa kwi-Eppendorftubes® 5.0ml, kunye ne-DNA / RNA kunye ne-DNA plasmid yokucoca isetyenziselwa ukuba ilula kakhulu. Ii-adapters zikwabonelelwa ukufezekisa i-centrifugation usebenzisa i-1.5 / 2 mL iityhubhu ze-centrifuge kunye ne-HPLC kunye ne-CRYO centrifugal tubes ngaphandle kokutshintsha i-rotor.

Iimpawu ze-5425R ze-centrifuge ezinesantya esiphezulu esinomkhenkce:
I-1, i-airtight EppendorfquickLock® cover, iisampuli zokuphatha zilula kakhulu, zikhuselekile;
I-2, iimpawu ze-software ezigqwesileyo (ezifana ne-adjustable acceleration and brake slope, 3 amaqhosha eprogram, akukho zinqumlo zamaqhosha aqhubekayo);
3, ukuphucula inkqubo ye-rotor (umzekelo, i-5.0ml rotor, ifanelekile kwinqanaba le-PCR pipe);
I-4, uyilo lwe-OptiBowl®, qinisekisa ukuba umsebenzi osisimumu, nokuba i-rotor cover nayo ithule kakhulu, yicinezele ngobumnene ukuvala i-centrifugal cover, akukho mandla anamandla kakhulu;
I-5, umthamo omkhulu we-rotor: 10 × 5.0ml ityhubhu ye-centrifuge
6, ubuninzi bamandla e-centrifugal: 21,300 × g (15,060 rpm)
I-7,5425R i-High Speed ​​​​Speed ​​​​Centrifuge imveliso ekhethekileyo yokusebenza, inkqubo yokupholisa ebalaseleyo yokusetyenzwa kwe-0.2-5 mL iisampulu ezibuthathaka zobushushu, itekhnoloji ye-compressor enelungelo elilodwa lomenzi, inciphisa ukungcangcazela, ukukhusela iisampulu. Uluhlu lobushushu: -10 c ukuya + 40 ° C.

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